antibody against ccna2  (Cell Signaling Technology Inc)

Journal: OncoTargets and Therapy

Article Title: Exploring Potential Biomarkers and Molecular Mechanisms of Cutaneous Squamous Cell Carcinoma Based on Bioinformatics

doi: 10.2147/OTT.S468399

Figure Lengend Snippet: Key Genes Identified Using the cytoHubba Plugin

Article Snippet: The tissue sections were incubated in 0.1% BSA, followed by overnight incubation at 4°C with antibodies against CCNA2 (Proteintech, 66087-1-Ig), CCNB2 (Proteintech, 66391-1-Ig), and UBE2C (Proteintech, 66726-1-Ig).

Techniques:

Journal: OncoTargets and Therapy

Article Title: Exploring Potential Biomarkers and Molecular Mechanisms of Cutaneous Squamous Cell Carcinoma Based on Bioinformatics

doi: 10.2147/OTT.S468399

Figure Lengend Snippet: Expression patterns of CCNA2, CCNB2, UBE2C in cSCC and normal tissues. ( a ) Protein levels of CCNA2, CCNB2, UBE2C in cSCC and normal tissues; ( b ) Expression of CCNA2, CCNB2, UBE2C in 30 pairs of cSCC and adjacent normal tissues, *** P < 0.001.

Article Snippet: The tissue sections were incubated in 0.1% BSA, followed by overnight incubation at 4°C with antibodies against CCNA2 (Proteintech, 66087-1-Ig), CCNB2 (Proteintech, 66391-1-Ig), and UBE2C (Proteintech, 66726-1-Ig).

Techniques: Expressing

Journal: OncoTargets and Therapy

Article Title: Exploring Potential Biomarkers and Molecular Mechanisms of Cutaneous Squamous Cell Carcinoma Based on Bioinformatics

doi: 10.2147/OTT.S468399

Figure Lengend Snippet: CeRNA networks. ( a ) The mRNA-miRNA co-expressed networks constructed by Cytoscape; ( b ) The ceRNA networks constructed by Cytoscape; ( c ) NEAT1/H19-hsa-miR-148a-3p-CCNA2 and NEAT1-hsa-miR-140-3p-UBE2C.

Article Snippet: The tissue sections were incubated in 0.1% BSA, followed by overnight incubation at 4°C with antibodies against CCNA2 (Proteintech, 66087-1-Ig), CCNB2 (Proteintech, 66391-1-Ig), and UBE2C (Proteintech, 66726-1-Ig).

Techniques: Construct

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Anticancer Action of Xiaoxianxiong Tang in Non-Small Cell Lung Cancer by Pharmacological Analysis and Experimental Validation

doi: 10.1155/2021/9930082

Figure Lengend Snippet: Information on 20 core targets.

Article Snippet: Primary antibodies against FOSL2 (cat. #: 15832-1-AP), α -tubulin (cat. #: 11224-1-AP), and GAPDH (cat. #: 10494-1-AP) were from Proteintech, and CCNA2 (cat. #: 4656) was from Cell Signaling Technology.

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Anticancer Action of Xiaoxianxiong Tang in Non-Small Cell Lung Cancer by Pharmacological Analysis and Experimental Validation

doi: 10.1155/2021/9930082

Figure Lengend Snippet: The cytotoxic effects of XXXT on lung cancer cells, RT-qPCR array, and western blot for core targets. (a) The inhibition rates of H460 and A549 cells treated with XXXT (0–1000 μ g/mL) for 72 h were determined using CCK8 assay. A549 or H460 treated with XXXT at IC 50 for 72 h were harvested for analysis (b–e). (b) Heatmap of 20 core targets mRNA expression in the control group and XXXT treatment group. The significant difference of core targets in A549 and H460 cells (c) was observed, including expression of CCNA2 and FOSL2 mRNA. The protein levels of CCNA2 and FOSL2 were determined by western blot (d). Statistical analysis of CCNA2 and FOSL2 proteins expression intensity (e). The above data are presented as mean ± SD for three independent experiments. NS, not significant, ∗ P < 0.05 and ∗∗ P < 0.01 showed significant difference vs. the control group.

Article Snippet: Primary antibodies against FOSL2 (cat. #: 15832-1-AP), α -tubulin (cat. #: 11224-1-AP), and GAPDH (cat. #: 10494-1-AP) were from Proteintech, and CCNA2 (cat. #: 4656) was from Cell Signaling Technology.

Techniques: Quantitative RT-PCR, Western Blot, Inhibition, CCK-8 Assay, Expressing, Control

Journal: Oncology reports

Article Title: BAI1‑associated protein 2‑like 2 is a potential biomarker in lung cancer.

doi: 10.3892/or.2018.6883

Figure Lengend Snippet: Figure 7. Multiple signaling pathways and proteins are regulated by BAIAP2L2 knockdown. (A) Gene‑Chip results revealed that 1,240 genes were upregulated and 1,157 genes were downregulated after BAIAP2L2 knockdown. (Criteria P<0.05, absolute fold change >2). (B) Pathway enrichment analysis was deter- mined by IPA software. (C) qRT‑PCR and western blot results revealed that CCNA2, CCND1 and CDK6 were downregulated and CDKN1B was upregulated in shBAIAP2L2 A549 cells. BAIAP2L2, BAI1‑associated protein 2‑like 2. *P<0.05.

Article Snippet: Antibodies against CCNA2 (dilution 1:1,000; cat. no. 4656), CDK6 (dilution 1:1,000; cat. no. 3136) and CDKN1B (dilution 1:1,000; cat. no. 3698) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Protein-Protein interactions, Knockdown, Software, Western Blot